Microbiology
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The Clinical Microbiology and Experimental Infectious Pathology Service is accredited by the University Aid and Research Management Agency (AGAUR). It has the following lines of research:
- Development, standardization and clinical evaluation of microbiological, immunological and molecular biology techniques: these procedures are used in the diagnosis of infectious diseases, the development of experimental models in infectious pathology, in the study of molecular mechanisms resistant to antimicrobials and the evaluation of antimicrobial activity of new antiseptics and disinfectants.
- Molecular virology and pathogenesis of viral infections, inflammation immunology, new bacterial anti-inflammatory molecules and epidemiology and public health of communicable diseases.
Apart from projects financed by public administrations through tender procedures, the group also has different R&D and technoloogy transfer contracts, projects and activities with the industry.
The research activities carried out by the Experimental Tuberculosis Unit can be consulted in its blog..
Presentation:
The Microbiology Services diagnoses different syndromes and infectious processes, in which the cause is significant to treatment. It also performs in vitro susceptibility studies of isolated microorganisms, along with studies of the antimicrobial spectrum of new antibiotics. It also deals with the detection and follow-up of resistance to antimicrobials, and actively participates in the detection, follow-up and control of hospital-acquired infections and in the design of the Germans Trias antibiotics policy.
It acts as a support laboratory of the Generalitat de Catalunya (Catalan Regional Government) in tuberculosis and in sexually transmitted infections (STI), in collaboration with CEEISCAT. Since 2001, the service has the Quality management certificate in accordance to standard ISO 9001:2000, and since 2009, in accordance to standard ISO 9001:2008.
The service has given accredited teaching to Microbiology residents since 1991. It has teaching responsibilities for undergraduates and postgraduates of the Medical School and Biomedical Science School of Universitat Autònoma de Barcelona (UAB).
A multidisciplinary research team has been formed around the service, established as a quality research group by the Generalitat de Catalunya since 1998 until today. Some members of the group form part of the CIBER of Respiratory Diseases (CIBERES) and others of the CIBER of Epidemiology and Public Health (CIBERESP) of the Instituto de Salud Carlos III.
Tipus Servei: 6
Correu: microbiologia.germanstrias@gencat.cat
Docència:
Undergraduate teaching
The subject of medical Microbiology and Parasitology is given to Medical and Biomedical Science degree students of Universitat Autònoma de Barcelona (UAB).
Postgraduate teaching
The service has been accredited to train resident doctors (of Medicine, Biology and Pharmacy) in the speciality of Microbiology and Parasitology since 1991. You can see all teaching guides in this link.
It also gives the microbiology module of the Master Degree in clinical Research applied to Health Sciences, in the Medical School of the UAB, and the doctorate programme in Microbiology of the Department of Genetics and Microbiology of the UAB.
Continuous training
Laboratory professionals give the annual refresher course in clinical Microbiology, open to all hospital staff and accredited by the Catalan Council of Continuous Training of Health Professions.
Medical and research team
Head of Service
Specialist
Specialist
Biologist
Specialist
Specialist
Specialist
Biologist. Senior technician
Pharmacist
Specialist
Specialist
Specilaist pharmacist
Resident biologist
Resident pharmacist
Nona Rodés Romaní
Resident
Researcher. Head of the Experimental Tuberculosis Unit (UTE) OF Institut Germans Trias
Jose Antonio Domínguez Benítez
Researcher of the National Health System
Miguel Servet Researcher at the National Health System
Miguel Servet Researcher at the National Health System
CIBER Respiratory Researcher
Intern of Institut Germans Trias
Predoctoral intern - Ministry of Education of Brazil
EDCTP predoctoral intern
Predoctoral student of the Experimental Tuberculosis Unit
Jorge Díaz Pedroza
Technician responsible for the P3 animal facility of the Experimental Tuberculosis Unit
Ismael Varela
Technician of the P3 animal facility of the Experimental Tuberculosis Unit
Technician and nursing team
Carmen Ramil Hernández
Technician and nursing team supervisor
Esmeralda Campaña García
Nursing team
Federico Cuchillo Canavate
Nursing team
Miguel Angel Cuesta Alonso
Nursing team
Gregorio Fernández Jacobe
Nursing team
Miguel Pérez Díaz
Nursing team
Montserrat Morilla Iglesias
Nursing team
Anna Cases Gomez
Nursing team
Rosa Tenllado Giménez
Nursing team
Elena Fernández Diaz
Nursing team
Mireya Alcaide Jorda
Technician
Maria Carrasco Díaz
Technician
Ma del Carmen Colomer López
Technician
Lucía Haba Giménez
Technician
Jordi Hernández Bolaños
Technician
Noelia López González
Technician
Ma Dolores Sánchez Muñoz
Technician
Jessica Hidalgo Heredia
Technician
Yaiza Rosales Salgado
Technician
Elisabet Bascuñana Prieto
Technician
Vanesa Barragan López
Technician
Marina Navarro Albareda
Technician
Adoración Velilla Pina
Technician
Neus Sanchez Luengo
Technician
Nuria Insa Sanchez
Technician
Ana Mata Jiménez
Technician
Natalia Pagan Maldonado
Technician
Cristina Sanchez-Lafuente Doncel
Technician
Nerea Gallardo Ruíz
Technician
Georgina Linares Llanas
Technician
Andrea Tiburcio Lara
Technician
Llibeth Arias de la Cruz
Predoctoral student
Administrative team
Isabel Carrasco Díaz
Administrative team
Aroa Muñoz Quinto
Administrative team
Cristina Marcos Guisado
Administrative team
Laura Villegas Bullón
Administrative team
The Microbiology Services diagnoses different syndromes and infectious processes, in which the cause is significant to treatment. It also performs in vitro susceptibility studies of isolated microorganisms, along with studies of the antimicrobial spectrum of new antibiotics. It also deals with the detection and follow-up of resistance to antimicrobials, and actively participates in the detection, follow-up and control of hospital-acquired infections and in the design of the Germans Trias antibiotics policy.
It acts as a support laboratory of the Generalitat de Catalunya (Catalan Regional Government) in tuberculosis and in sexually transmitted infections (STI), in collaboration with CEEISCAT. Since 2001, the service has the Quality management certificate in accordance to standard ISO 9001:2000, and since 2009, in accordance to standard ISO 9001:2008.
The service has given accredited teaching to Microbiology residents since 1991. It has teaching responsibilities for undergraduates and postgraduates of the Medical School and Biomedical Science School of Universitat Autònoma de Barcelona (UAB).
A multidisciplinary research team has been formed around the service, established as a quality research group by the Generalitat de Catalunya since 1998 until today. Some members of the group form part of the CIBER of Respiratory Diseases (CIBERES) and others of the CIBER of Epidemiology and Public Health (CIBERESP) of the Instituto de Salud Carlos III.
- Serology of HIV+2 by EIA technique
- Confirmatory test for immunoblot (IMB) and quantitative detection of viral RNA (viral load)
- Diagnosis of recent infection by RITA algorithm (recent infection testing algorithm)
- Control and follow-up of antiretroviral therapy
- Serology of hepatitis B virus (HBV) in acute and chronic infection, by immunoenzymatic assay technique (EIA)
- Quantitative detection of DNA in HBV
- Follow-up and control of specific treatment
- Serology of hepatitis C virus (HCV) by EIA technique
- Confirmatory IMB test
- Quantitative detection of RNA in HCV
- Genotyping and subtyping of HCV
- Diagnosis of acute infection and control of response to hepatitis C treatment
- Diagnosis and monitoring of other viral hepatitis
- Conventional culture techniques for microorganisms causing sexually transmitted infections (STI)
- Nucleic acid detection techniques for STI causal agents (clamidia and gonococcal infections)
- Diagnosis and follow-up of syphilis
- Nucleic acid detection techniques for the diagnosis of other STI causal agents
- Conventional culture techniques for microorganisms causing acute, post-operative infections or on prosthetic material at osteoarticular level
- Nucleic acid detection techniques for the main causal agents of acute arthritis and osteomyelitis
- Infection of upper respiratory tract: conventional culture techniques to diagnose bacterial infection, techniques to detect antigen and nucleic acid detection to diagnose viral infections
- Infections of lower respiratory tract: conventional or quantitative culture techniques for lower respiratory tract invasive specimens. Antigen detection techniques in urine for the diagnosis of bacterial pneumonia. Nucleic acid detection techniques to diagnose bacterial or viral infection. Infection diagnosis in patients subject to mechanical ventilation
- Screening of urine specimens by flow cytometry
- Conventional culture techniques to identify the bacteria responsible for urinary tract infections (UTI)
- Direct examination and conventional culture for causal agents of meningitis
- Antigen detection techniques for the diagnosis of bacterial and fungal infections
- Nucleic acid detection techniques to diagnose bacterial, viral and parasitic infections causing menigoencephalitis
- Conventional culture techniques for the diagnosis of bacterial infections
- Nucleic acid detection techniques to diagnose bacterial, viral and parasitic infections causing menigoencephalitis
Automated techniques of blood cultures in adults and children
Quick identification and study techniques of in vitro susceptibility of isolated microorganisms
- Conventional culture techniques of antigen and nucleic acid detection to identify the main bacteria and virus causing gastroenteritis
- Direct examination through observing protozoa and other parasites causing gastrointestinal infections
Direct examination of antigen and specific culture detection to research imported parasitosis
- Determination of sensitivity patterns of isolated gram-positive and gram-negative bacteria in cultures by disc diffusion technique and by CMI
- Surveillance and integration in daily clinical practice of molecular methods to detect resistance mechanisms in the main multi-resistant microorganisms
- Evaluation of the use of new quick diagnosis molecular methods to detect resistant microorganisms
Molecular typing techniques to compare the genetic outline of isolated strains in infectious outbreaks.
Evaluation of new candidates of anti-tuberculosis vaccinations or drugs, or of other infectious diseases in experimental models, mainly animals.
Ethiologic diagnosis of sepsis
Sepsis is the most common cause of admission to hospital, more than a heart attack, with a higher death rate. A patient with sepsis is a serious case, with inflammatory response throughout the body, and has a known or suspicious infection. It is calculated that in Spain, there are over 50,000 cases of sepsis a year, of which up to a third lead to death.
For the correct care of these patients, a quick ethiologic diagnosis (finding out the causes) is one of the keys to the process. Today's blood culture systems have a response time that is still too long, although Germans Trias apply the most cost-effective processing techniques available.
In the short-term, we will be in a position to apply new systems (IRIDICA system, Abbot molecular), which will enable us to identify the causative agents of sepsis in a tenth of the time. It will also detect the main resistance mechanisms to antimicrobials, which will make it possible to apply the most cost-effective antibiotic treatment.
Diagnosis of tuberculous infection and disease
Tuberculosis is a worldwide health problem. According to the WHO, in 2012 there were 8.6 million patients, and 1.3 million deaths. In recent years, its treatment has become even more complicated, owing to the irruption of multi and extremely resistant bacteria strains: in 2012, 450,000 cases of multiresistant cases arose, of which 9.6 were extremely resistant.
The correct identification of tuberculosis cases, with the resistance pattern of the strains, is an essential tool in successfully fighting this disease. For the diagnosis of the infection, the laboratory can carry out IGRAS tests, the sensitivity and specificity of which are an important asset of the process. In recent years, detection systems have been implemented of nucleic acids, which at the same time detect the tuberculosis bacillus and its resistance to rifampicin. These tests, with fast results, enable us to quickly start or redirect anti-tuberculosis treatment, hence leading to its success.
The Clinical Microbiology and Experimental Infectious Pathology Service is accredited by the University Aid and Research Management Agency (AGAUR). It has the following lines of research:
- Development, standardization and clinical evaluation of microbiological, immunological and molecular biology techniques: these procedures are used in the diagnosis of infectious diseases, the development of experimental models in infectious pathology, in the study of molecular mechanisms resistant to antimicrobials and the evaluation of antimicrobial activity of new antiseptics and disinfectants.
- Molecular virology and pathogenesis of viral infections, inflammation immunology, new bacterial anti-inflammatory molecules and epidemiology and public health of communicable diseases.
Apart from projects financed by public administrations through tender procedures, the group also has different R&D and technoloogy transfer contracts, projects and activities with the industry.
The research activities carried out by the Experimental Tuberculosis Unit can be consulted in its blog..
Undergraduate teaching
The subject of medical Microbiology and Parasitology is given to Medical and Biomedical Science degree students of Universitat Autònoma de Barcelona (UAB).
Postgraduate teaching
The service has been accredited to train resident doctors (of Medicine, Biology and Pharmacy) in the speciality of Microbiology and Parasitology since 1991. You can see all teaching guides in this link.
It also gives the microbiology module of the Master Degree in clinical Research applied to Health Sciences, in the Medical School of the UAB, and the doctorate programme in Microbiology of the Department of Genetics and Microbiology of the UAB.
Continuous training
Laboratory professionals give the annual refresher course in clinical Microbiology, open to all hospital staff and accredited by the Catalan Council of Continuous Training of Health Professions.
Contacte: microbiologia.germanstrias@gencat.cat